Synthesis of 3-carbethoxy-4(3’-chloro-4’-hydroxy) phenyl-but-3-en-2-one and its cytotoxicity evaluation against cancer cell carrying mutant p53

Overexpression of mutant p53 in cancer cell inactivates the pathways to execute apoptosis and cycle arrest. This study aimed synthesize new kardiena derivative compound, 3-carbethoxy-4(3’-chloro-4’-hydroxy)phenyl-but-3-en-2-one, reactivate Characterization synthetic compound employing melting point, IR, EI-MS, 1H-NMR, 13C-NMR spectra revealed 3-carbethoxy-4(3’-chloro-4’-hydroxy)phenyl-but-3-en-2-one was successfully synthesized from 3-chloro-4-hydroxybenzaldehyde ethyl acetoacetate using dimethylamine as a catalyst. had antiproliferative activity against WiDr cells which carried p53. Its better than 5’-FU reference standard treat colon cancer. Increasing accumulation G2-M phase, active form caspase-3, inducing demonstrated ability arrest carrying